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cloning vector pcmv xl5  (OriGene)


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    OriGene cloning vector pcmv xl5
    Cloning Vector Pcmv Xl5, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1431 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cloning vector pcmv xl5/product/OriGene
    Average 96 stars, based on 1431 article reviews
    cloning vector pcmv xl5 - by Bioz Stars, 2026-03
    96/100 stars

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    Reporter and expression vectors were used in transient transfection assays in HEK293 cells as indicated in materials and methods. Reporter vector in which the firefly luciferase gene is under the control of 3 copies of wild type (3xwt-κB) or mutant (3xmut-κB) NF-κB binding was cotransfected with HDAC4 expression vector. pCMV was used a control empty vector. 24 hours post-transfection, cells were treated with 25 ng/ml of TNF-α for overnight and then, the luciferase activity was carried out as described in materials and methods. Protein concentration was used for normalization.

    Journal: PLoS ONE

    Article Title: Proteomics Analysis of Human Obesity Reveals the Epigenetic Factor HDAC4 as a Potential Target for Obesity

    doi: 10.1371/journal.pone.0075342

    Figure Lengend Snippet: Reporter and expression vectors were used in transient transfection assays in HEK293 cells as indicated in materials and methods. Reporter vector in which the firefly luciferase gene is under the control of 3 copies of wild type (3xwt-κB) or mutant (3xmut-κB) NF-κB binding was cotransfected with HDAC4 expression vector. pCMV was used a control empty vector. 24 hours post-transfection, cells were treated with 25 ng/ml of TNF-α for overnight and then, the luciferase activity was carried out as described in materials and methods. Protein concentration was used for normalization.

    Article Snippet: The expression vector encoding HDAC4 (pCMV-HDAC4) and the pCMV empty vector were purchased from OriGene (OriGene Technologies, Inc, Rockville, MD).

    Techniques: Expressing, Transfection, Plasmid Preparation, Luciferase, Mutagenesis, Binding Assay, Activity Assay, Protein Concentration